Standard Deviation and Standard Error – What is the Difference?

When studying results of scientific publications one usually comes across standard deviations (SD) and standard errors (SE). However, even both measures are widely used the difference between them is not always clear to the readers. This article aims to clarify some important points and to provide a deeper understanding of SD and SE.

When conducting a study, specific variables (e.g. age, blood pressure) in a sample are collected. This sample has been taken from a larger population of interest, the so-called total population. Such a total population might comprise for example all patients with a specific indication. Ideally, the sample taken within the study represents the total population, i.e. it is similar to the total population with respect to collected data.

The SD gives the variability of a variable within the study sample providing an estimate of the actual variability in the total population. For example, a total population with a heterogeneous age distribution should also lead to a high SD of age in the study sample. It is calculated as the average of the (quadratic) differences from the sample (arithmetic) mean \overline{x}:

\large SD=\sqrt{\frac{\sum^n_{i=1}{(x_i-\overline{x})^2}}{n-1}}

For interpretations, it is important to know: \overline{x}\pm SD overs about 68% and \overline{x}\pm 2\cdot SD about 95% of all observations in the sample regardless of the distribution. Moreover, the SD is strongly influenced by single outliers. That means, few extreme high or low values can increase SD remarkably.

The SE on the other side depends on the measurement accuracy of the variable and gives the precision of the sample mean with respect to the mean in the total population. To understand this it is important to remember that the sample mean itself is only an estimation with a specific uncertainty: different study samples will yield to different sample means. In other words, the SE gives the precision of the sample mean.

However, there is a direct connection between SE and SD:

\large SE=SD/\sqrt{n}

Hence, the SE is always smaller than the SD and gets smaller with increasing sample size. This makes sense as one can consider a greater specificity of the true population mean with increasing sample size. In contrast, the SD, which estimates the variability in the total population is independent from the sample size, i.e. it remains similar when increasing the sample size.

An example of SD and SE of age for two fictive populations is given in the table. The first population comprises six patients. The second population comprises twice population 1. Thus, the mean and SD are identical in both populations. However, as the SE depends on the number of patients the SE of population 2 is smaller than of population 1.

Table: Example for SD and SE of age in two populations.

Possibly because the SE is always smaller than the SD, many authors tend to show SE in order to describe the variability of a variable. However, this is not correct. Actually, the SE should only be used for hypothesis testing (e.g. “Is the actual mean different from a specific constant value?” or “Are means of two groups different?”) and to calculate confidence intervals (CI):

\large 95\%-CI=\overline{x}\pm 1.96\cdot SE

In summary, when describing the variability of measurements in a sample, the SD is the parameter of choice. The SE describes the uncertainty of the sample mean and it should only be used for inferential statistics (hypothesis testing and confidence intervals).


Altman D. and Bland M.: Standard deviations and standard errors. BMJ 2005; 331

Koschack J.: Standardabweichung und Standardfehler: der kleine, aber feine Unterschied. Z Allg Med 2008; 84

Nagele P.: Misuse of standard error of the mean (SEM) when reporting variability of a sample. A critical evaluation of four anaesthesia journals. Br J Anaesth. 2003 Apr; 90 (4)

Picture: © ISTANBUL2009


Get the latest articles as soon as they are published: for practitioners in clinical research

  • Read about ideas & tools for effective clinical research

  • Follow today’s topics in clinical research

  • Knowledge base: study design, study management, digitalization & data management, biostatistics, safety

  • It’s free! Sign up now!

We use the Google service reCaptcha to determine whether a person or a computer makes a specific entry in our contact or newsletter form. Google uses the following information to determine if you are a human being or a computer: IP address of the terminal device you are using, the website you are visiting and on which the captcha is integrated, the date and duration of the visit, the identification data of the browser and operating system type used, Google account if you are logged in to Google, mouse movements on the reCaptcha areas and tasks for which you must identify images. The legal basis for the described data processing is Art. 6 para. 1 lit. f General Data Protection Regulation. There is a legitimate interest on our part in this data processing to ensure the security of our website and to protect us from automated input (attacks).